最先端研究セミナー

講演者：　斎藤　通紀（京都大学大学院医学研究科　生体構造医学講座　教授）

演題：　Mechanism and Reconstitution In Vitro of Mammalian Germ Cell Development

Abstract:

The germ cell lineage ensures the creation of new individuals, perpetuating/diversifying the genetic and epigenetic information across the generations. We have been investigating the mechanism for germ cell development, and have shown that mouse embryonic stem cells (mESCs)/induced pluripotent stem cells (miPSCs) are induced into primordial germ cell-like cells (mPGCLCs) with a robust capacity both for spermatogenesis and oogenesis and for contributing to offspring.  These works have served as a basis for exploring the mechanism of key events during germ cell development such as epigenetic reprogramming and sex determination/meiotic entry.  We have also shown that human iPSCs (hiPSCs) with a primed pluripotency robustly generates human PGCLCs (hPGCLCs) with a property of human early PGCs.  Moreover, by investigating the development of cynomolgus monkeys, we have defined a developmental coordinate of the spectrum of pluripotency among mice, monkeys, and humans, and have identified the origin of the germ cell lineage in cynomolgus monkeys in the amnion.  More recently, we have shown the hPGCLCs can be induced into oogonia/early oocytes with appropriate epigenetic reprogramming in xenogenetic reconstituted ovaries.

I would here like to discuss our latest findings regarding the mechanism of and reconstitution in vitro of germ cell development in mice, monkeys, and humans.

担当分野： 脳発生　嶋村（内線：6583）

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