Cutting edge Seminar

 

Speaker:  Yoichi shinkai ph.D., (Chief Scientist, Cellular Memory Laboratory, RIKEN)

Title: Epigenetic regulation of transposable element

 

 

 

Date&Time:  15 Feb. (Wed.) 2017, 12:00- 13:00
Venue: Conference Room(1F), IMEG

Abstract:

Transposon-derived elements (TEs) comprise approximately 40% of the mouse genome and retroelements including LINE, SINE and endogenous retroviruses (ERVs) are capable of active transposition. Although transposition contributes to genome diversification and evolution, transpositions at a high frequency could cause genome instability and are deleterious to host species. Therefore, hosts have equipped multiple defense mechanisms against transposition(1). Transcriptional silencing is the first line of the defense mechanisms against TEs integrated into the genome and various epigenetic machineries such as DNA methylation and histone methylation play important roles in TE silencing(2-9). In order to catalog genes involved in ERV silencing and advance our understanding on retroelement silencing, we here performed a CRISPR-gRNA-based genome-wide screen in mESCs carrying a GFP-reporter provirus. In addition to known genes involved in retroelement silencing, our screen identified a number of previously uncharacterized genes. In my talk, I’d like to describe what kind of genes are identified and discuss how they are integrated in the provirus silencing.

 

References

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