講演者：　眞貝　洋一　（理化学研究所　眞貝細胞記憶研究室　主任研究員）

演題：　Epigenetic regulation of transposable element

日時： 2月15日（水） 12:00-13:00
会場： 発生医学研究所1階　カンファレンス室

Abstract:

Transposon-derived elements (TEs) comprise approximately 40% of the mouse genome and retroelements including LINE, SINE and endogenous retroviruses (ERVs) are capable of active transposition. Although transposition contributes to genome diversification and evolution, transpositions at a high frequency could cause genome instability and are deleterious to host species. Therefore, hosts have equipped multiple defense mechanisms against transposition(1). Transcriptional silencing is the first line of the defense mechanisms against TEs integrated into the genome and various epigenetic machineries such as DNA methylation and histone methylation play important roles in TE silencing(2-9). In order to catalog genes involved in ERV silencing and advance our understanding on retroelement silencing, we here performed a CRISPR-gRNA-based genome-wide screen in mESCs carrying a GFP-reporter provirus. In addition to known genes involved in retroelement silencing, our screen identified a number of previously uncharacterized genes. In my talk, I’d like to describe what kind of genes are identified and discuss how they are integrated in the provirus silencing.

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担当分野：多能性幹細胞分野　丹羽仁史（内線：6620)

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