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講演者: 鐘巻 将人(国立遺伝学研究所 分子細胞工学研究室 教授)

演題: Controlling protein expression by using the power of plants



日時: 2021年4月21日(水)12:00- 13:00

会場: 発生医学研究所1階 カンファレンス室

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Genetic perturbation is a powerful way to analyze the function of proteins in living cells. For this purpose, we pioneered to develop the auxin-inducible degron (AID) technology by which a degron-fused protein can be rapidly degraded after the addition of the plant hormone auxin (1). By combining with CRISPR-based genome editing, it was possible to generate AID conditional mutants of human cells (2). The AID system became one of the popular genetic tools to study the function of proteins. However, leaky degradation and high doses of auxin for inducing degradation have been major drawbacks. Moreover, nobody has successfully applied the AID system to control protein degradation in living mice. We recently overcame these problems by taking advantage of chemical biology and successfully established the AID2 system (3). By using AID2, we can now sharply control protein degradation in yeast, mammalian cells and mice.


1. Nishimura et al. (2009) An Auxin-based Degron System for the Rapid Depletion of Proteins in Nonplant Cells. Nature Methods, 6, 917-922.
2. Natsume et al. (2016) Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Reports, 15, 210-218.
3. Yesbolatova et al. (2020) The Auxin-Inducible Degron 2 Technology Gives Sharp Degradation Control in Yeast, Mammalian Cells, and Mice. Nature Communications, 11, 5701.


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