2022-09-21

 

 

 

Abstract:

DNA replication is the mechanism underlying genetic inheritance and its accuracy and efficiency are essential for all organisms. In humans, the loss of replication fidelity is responsible for both inherited syndromes and somatic disease such as cancer. There are 16 different DNA polymerases in eukaryotes and the fidelity and efficiency of their synthetic activities are distinct. The correct division of labour among these polymerases is therefore a primary factor in determining the stability of genome duplication. Studies in budding yeast and fission yeast previously demonstrated that Polε is assigned for leading strand DNA synthesis and Polδ in mammalian genomes has not been explored. We therefore aimed to identify a genome-wide profile of replicative DNA polymerase in human cultured cells. Furthermore, Using the genome profiles of replicative polymerase, we currently investigate how replication kinetics are governed by various genomic features.

 

 

Reference: 

Koyanagi et al BioRxiv, https://doi.org/10.1101/2021.11.14.468503, 2021

Daigaku at al PLoS Genet. 13 e1006789 2017

Daigaku at al 22 Nat. Struct. Mol. Biol.192-198 201

 

 

 

 

 

担当分野：損傷修復　立石（内線：6605）