最先端研究セミナー

講演者：栗崎　晃（奈良先端科学技術大学院大学先端科学技術研究科　バイオサイエンス領域　幹細胞工学研究室　教授）

演題：Mechanisms of gastric tissue differentiation and the control of homeostasis

日時：2025年11月5日（水）　12：00-13：00

開催場所：発生医学研究所1階　カンファレンス室

※ZOOMミーティングのURLはMoodleの「S-HIGO最先端研究セミナーA、B」にてご確認ください。

https://md.kumamoto-u.ac.jp/course/view.php?id=120331

Abstract：

The adult stomach performs digestive functions through various
specialized cell types, including parietal cells that secrete gastric
acid, chief cells that produce digestive enzymes, and pit cells that
secrete large amounts of mucus to protect the luminal surface of the
stomach lining. We established a differentiation culture system that
enables the generation of gastric mini-tissues from pluripotent stem
cells, providing an in vitro model to investigate the mechanisms
underlying gastric differentiation.

Furthermore, focusing on stem cells in the adult stomach, we explored
candidate signaling pathways regulating the differentiation of adult
gastric epithelial stem cells by performing pseudotime-dependent gene
enrichment analysis of single-cell RNA sequencing data from adult
gastric tissue. Using these candidate factors, we conducted
differentiation assays on gastric epithelial stem cells isolated and
expanded from adult gastric tissue. Our results revealed that EGFR–ERK
signaling promotes differentiation into pit cells, whereas NF-κB
signaling maintains gastric progenitor cells in an undifferentiated state.

In this seminar, I will introduce these mechanisms controlling gastric
differentiation and discuss the fundamental principles underlying
gastric epithelial homeostasis.

担当分野：遺伝子機能応用学　首藤（4407)