Cutting edge Seminar

 

Speaker:  Katsuyuki Yugi  (Team Leader, Laboratory for Integrated Cellular Systems, RIKEN Center for Integrative Medical Sciences)

Title: 　Trans-omics: integration of multiple omic data on the basis of reaction kinetics

 

Date&Time:  14 Jun.  (Wed.) 2021, 12:00- 13:00

※This seminar can also be attended through ZOOM. Please check the URL on “HIGO Cutting-Edge Seminar” at Moodle.

https://md.kumamoto-u.ac.jp/course/view.php?id=90416

 

Abstract:

Metabolic homeostasis is realized by global networks of molecular interactions across multiple ‘omic’ layers such as genome, transcriptome, proteome, and metabolome. We have proposed ‘trans-omics’, a methodology to reconstruct large-scale networks that span across the multiple omic layers on the basis of time-series omic data and reaction kinetics [1,2]. Here we show an application of trans-omics to reconstruction of a metabolic regulatory network that underlies acute insulin action in rat hepatoma FAO cells [3].  The network integrates phosphoprotome and metabolome data, and involves 13 protein kinases, 26 phosphorylated metabolic enzymes, and 35 allosteric effectors, resulting in quantitative changes in 44 metabolites. Mathematical modeling analysis predicted selective control of a subnetwork around phosphofructokinase by specific phosphorylation and allosteric regulation. Overall, we provide an unbiased method that reconstructs the trans-omic network from phosphoproteome and metabolome data, which will be applicable to other cellular responses.

 

Fig.1. A global landscape of the trans-omic network of acute insulin action in rat hepatoma FAO cells [3].