2022-09-21
※ This seminar has been cancelled.
Cutting edge Seminar
Speaker: Yasukazu Daigaku (Project Leader, Cancer Genome Dynamics Project, The Cancer Institute for
Japanese Foundation for Cancer Research)
Title: Genome-wide profiling of DNA polymerase usage and replication kinetics in human cells
Date&Time: 21 Sep. (Wed.) 2022, 12:00- 13:00 Cancelled
※This seminar can also be attended through ZOOM. Please check the URL on “HIGO Cutting-Edge Seminar” at Moodle.
https://md.kumamoto-u.ac.jp/course/view.php?id=95315
Abstract:
DNA replication is the mechanism underlying genetic inheritance and its accuracy and efficiency are essential for all organisms. In humans, the loss of replication fidelity is responsible for both inherited syndromes and somatic disease such as cancer. There are 16 different DNA polymerases in eukaryotes and the fidelity and efficiency of their synthetic activities are distinct. The correct division of labour among these polymerases is therefore a primary factor in determining the stability of genome duplication. Studies in budding yeast and fission yeast previously demonstrated that Polε is assigned for leading strand DNA synthesis and Polδ in mammalian genomes has not been explored. We therefore aimed to identify a genome-wide profile of replicative DNA polymerase in human cultured cells. Furthermore, Using the genome profiles of replicative polymerase, we currently investigate how replication kinetics are governed by various genomic features.
Reference:
Koyanagi et al BioRxiv, https://doi.org/10.1101/2021.11.14.468503, 2021
Daigaku at al PLoS Genet. 13 e1006789 2017
Daigaku at al 22 Nat. Struct. Mol. Biol.192-198 201
